They are prompt in replying to my emails and very helpful with their advice. Thanks a lot! Vahan Serobyan, Prof. provided by Diagenode to perform western blots.The high level of specificity of these antibodies in Pristionchus pacificus samples, confirmed by using several negative and positive controls run in parallel with synchronized culture samples, ensured successful and reproducible results.Īlso, I am satisfied with the service and the support of personnel of Diagenode. I have used the antibodies against the histone modifications H3K4me3, H3K4me2, H3K4me1, H3k27me3, H3K9ac, H3K27ac etc. I have been a Diagenode customer for over two years now. The high sensitivity and specificity of our antibodies enable the most accurate results. New! Blue ladder monoclonal antibody is available Antibodies validated in WBĭiagenode offers the antibodies selected for highest-quality results in Western blot.
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This figure clearly demonstrates that the antibody does not react with any proteins of the species that were tested. The left figure shows a Ponceau staining of the gel. Protein extracts from different species were subjected to SDS-PAGE and analyzed by Western blot with the Diagenode Blue ladder - HRP antibody (Cat. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody Outstanding specificity with no cross-reactivity or background.Compatible with most blue-stained ladders.Develop your marker directly on X-ray film.Faster and easier protein detection - manual marking is no longer necessary.This makes the positioning of the marker and thus the accurate detection of proteins significantly easier. Diagenode has developed a revolutionary new antibody that specifically reacts with this blue dye, enabling direct visualization of the different marker fragments on the blot. Most prestained protein MW markers used in western blot contain fragments that are labelled with a blue dye. Learn more about this antibody Blue ladder-HRP monoclonal antibody Visualize your marker directly on film To check that the signal detection after antibody incubation is homogenous across the lines.To confirm that the electrotransfer was done equally across the lines.To verify that equal sample amount was loaded on the gel.The marker (in kDa) is shown on the left,and the position of the protein of interest is indicated on the right. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Note: The WesternSure Ladder is not recommended for use when stripping and re-probing Western blots. Western blot was performed on whole cell extracts (30 μg) from different cell types (lane 1: HeLa, lane 2: K562, lane 3: MCF7, lane 4: U2OS, lane 5: HepG2, lane 6: Jurkat, lane 7: NIH3T3, lane 8: E14Tg2a mouse ES cells) using the monoclonal antibody against H3. The WesternSure chemiluminescent protein ladder is the only pre-stained, multi-colored protein ladder for both film and digital chemiluminescence detection. (Unstained protein ladders have only the bromophenol blue at the dye front to help monitor progress it does not help to assess blotting efficiency.Western blot analysis using H3pan monoclonal antibody They offer easier identification of protein molecular weights and the ability to monitor migration during electrophoresis. These prestained recombinant protein ladders include three high-intensity reference bands: 25, 50, and 75 kD. Calculate the approximate size of your protein by comparing the image of the membrane with the ladder to the image produced by antibody staining.Verify transfer efficiency between the gel and PVDF, nylon, or nitrocellulose membranes.Consisting of recombinant proteins fused to an IgG binding site, these versatile molecular weight ladders bind the primary or secondary antibody used for the detection of the target protein. Monitor protein separation during SDS-polyacrylamide gel electrophoresis Western blot protein ladders are designed for protein molecular weight estimation directly on the blot during detection.In western blotting, prestained protein ladders can be used to: Each protein can be stained with a different color for easy identification or labeled with affinity tags for western blot detection. Recombinant protein ladders are engineered to produce tight bands, evenly spaced molecular weights, and other specific traits. Although some laboratories continue to use natural protein ladders, recombinant standards are more commonly used. These broad bands are less distinct, which makes them less useful for molecular weight estimation. Prestained natural proteins may produce broader bands than recombinant proteins, especially when they are stained with more than one color. Prestained naturally occurring proteins will vary due to the amount and location of dye binding sites.